Enlightening Proteomics

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Phenomics Australia is shedding new light on proteomics with the IsoLight system, an automated cellular proteomics hub.

The Victorian Centre for Functional Genomics, through partnership with Trend Bio, has recently installed the IsoLight instrument from IsoPlexis for single cell proteomics and bulk proteomics, with an emphasis on immuno-oncology applications.

The data from this instrument enables researchers to map the adaptive or innate immune landscape of human and mouse T cells, CART cells and NK cells by simultaneously measuring up to 32 different cytokines secreted by each single cell. It can perform fully automated multiplex ELISAs of up to 30 cytokines per sample in over 60 samples in one run. Lastly, researchers can identify tumour signalling pathways associated with drug resistance or metastasis by investigating intracellular phosphoproteins or metabolites expressed in single cells.

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