VCFG taking 3D to the next level with multiple imaging platforms

Share this:

Over the past year the VCFG has established a high throughput 3D screening platform using either 96 or 384 well plate format. This platform enables researchers to study cell linespatient- or PDX-derived material to understand kinetics of structure growth and formation, alongside responses to standard of care clinical treatment regimens as well as large scale drug discovery. Critically important is the capacity to co-culture structures with other cell types that are normally part of the cellular microenvironment, such as immune signalling subtypes and CAR-T cells. Using high content imaging as a predominant readout, the VCFG has shown the inherent value in capturing all the cellular information and has developed very sophisticated and customised analysis pipelines.  The VCFG make use of both label free and fluorescence labelled imaging scenarios to complete their large-scale screening projects, heavily utilising the Cytation 5 and Cellomic CX7-LZR platforms.

Message from our nodes:

A/Prof Kaylene Simpson head of the VCFG.

‘ We have continued to support screening projects throughout the COVID lockdown in Melbourne, albeit at a slower pace. During this time we have developed our automated 3D screening platform using cell linespatient derived organoids and PDX material. We have created sophisticated high content imaging and analysis pipelines for measuring 3D structure growth over time and in response to treatment with compounds, singularly or in combination to measure synergy.  Taking advantage of COVID, we have updated and written an extensive suite of SOPstechnologyinstrument and operational user guides. Importantly, we have implemented a comprehensive project management portal to future proof projects for today until publication.

Phenomics Australia Functional Genomics and High Throughput Screening operates as a partnership between the Victorian Centre for Functional Genomics (VCFG) at the Peter MacCallum Cancer Centre, the Harry Perkins Institute of Medical Research (Perkins), and newly through ANU Centre for Therapeutic Discovery (ACDT, The John Curtin School of Medical Research, ANU), Monash University, and the University of Adelaide (in partnership with SAHMRI) to perform genome-scale cell-based CRISPR, RNAi (directed to protein coding genes, miRNAs and long-non-coding RNAs) and compound screening using sophisticated liquid handling automation and underpinned by high content imaging in both 2D and 3D settings.

Phenomics Australia Feedback Form

Phenomics Australia (formerly the Australian Phenomics Network, APN) offers consolidated infrastructure and expertise supporting genomic medicine and biomedical research from discovery through into early clinical development and evaluation.

Phenomics Australia is a founding capability enabled by the National Collaborative Research Infrastructure Strategy (NCRIS). We would love to hear from you!

Please feel free to provide any ideas or general comments you might have so that we can further improve how Phenomics Australia can support you.

Phenomics Australia PHENOMENA News Contribution

Have you recently had a publication accepted? Do you want to tell us about a grant you have received? Or maybe you have just been recently in the news? You can complete this form to tell us about your latest research developments.

Information provided will be used to inform press releases, website stories, social media content and more. This form is being used to capture everything that is happening at Phenomics Australia, which can then be used as part of our outreach and communications.

Once you have completed the form, the Communications and Outreach Coordinator will then be in contact with you.


Make an Enquiry

_Get in touch to discuss how we can help with your research.