Have you ever thought of performing a cell killing assay without using radioactive materials?
Dr Sek tracked live tumour (adherent and non-adherent) cell growth over 48 hours in the presence or absence of CAR T cells. He was able to show that CAR T cells were effectively killing tumour cells over time. He captured images and videos of the whole process in 384-well plate format, allowing him to test several conditions in a single experiment. In comparison, if he performed the gold standard chromium killing assay, in a 96-well format, he would use substantially more cells, would be limited in the number of test conditions and would only have a single end point assay result.
This is such a great advance!
‘ Through our collaboration with Prescient Therapeutics, we wanted to determine the anti-tumour capabilities of Prescient’s new universal OmniCAR T cell platform. I teamed up with the VCFG staff who were critical in optimizing and setting up real-time, coculture experiments on the Incucyte. Using a small number of CAR T cells and tumour targets, I was able to obtain high quality videos and images, quantify tumour cell killing and T cell proliferation, and also determine multiple antigen targeting of the Universal OmniCAR T cells in a mixed tumour killing assay.”
The IncuCyte SX5, at the Victorian Centre for Functional Genomics (VCFG), is a live-cell imaging microscope that is housed inside a cell culture incubator. It allows researchers to image up to six 384-well plates in phase, green and red channels every 2 hours, in parallel! Its user friendly software can quantify cell number, size, shape and fluorescence intensity at the single-cell level over time.